Skip to main content
Go to the University of Minnesota Twin Cities home page
  • One Stop
  • MyU: For Students, Faculty, and Staff

Department of Otolaryngology-Head & Neck Surgery

Paparella Otopathology & Ear Pathogenesis Laboratory

Menu

Breadcrumb

  1. Home
  • Home
  • Research resources
  • Research collaborations
  • Publications
  • Staff
  • Fellows
  • National Ear Resource Network

Standard Procedures, Manuals, and Instruction Videos

Standard Procedures for Celloidin-Embedded Human Temporal Bones

A midmodiolar image of a cochlea stained with hematoxylin and eosin
  • General Laboratory Solutions (Celloidin, EDTA, H&E, Formalin, Citrate-Phosphate Buffer, 10% Sodium Hydroxide, Ammonium Oxalate)
  • Fixation, Decalcification, Dehydration, and Embedding
  • Hematoxylin and Eosin staining
  • Mounting Slides

 

Step-by-Step Processing Videos for Celloidin-Embedded Human Temporal Bones

A temporal bone embedded in celloidin being sectioned
  • Preparing Blocks for Sectioning: Step-by-step Video
  • Sectioning: Step-by-step Video
  • Staining Step-by-step Video
  • Mounting Slides and Cleaning: Step-by-step Video

 

Other types of Processing and Staining: Manuals and Protocols

Cochlear inner hair cell stereocilia
  • Immunohistochemistry protocol for staining hair cell cilia of mice cochleae (whole mount samples)
  • Animal temporal bone sectioning
  • Animal temporal bone staining

 

Imaging Protocols

A midmodiolar section of a temporal bone with extensive otosclerosis
  • Scanning Temporal Bone Slides With AxiosCan 7 (20x, Lossless Compression)

     

 

Contact information

email: [email protected]

For Students, Faculty, and Staff

  • One Stop
  • MyU: For Students, Faculty, and Staff
© 2026 Regents of the University of Minnesota. All rights reserved. The University of Minnesota is an equal opportunity educator and employer. Privacy Statement Report Web Accessibility Issues
  • Home
  • Research resources
  • Research collaborations
  • Publications
  • Staff
  • Fellows
  • National Ear Resource Network